Wahlmodul 31: Optophysiology

Lecturers: Prof. Dr. Ilka Diester, PD Dr. Philippe Coulon, Prof. Dr. Barbara Di Ventura, Dr. Franziska Schneider
Lecture: IMBIT, Georges-Köhler-Allee 201, 79110 Freiburg
Practical Course
Experiments 1-3: IMBIT, Georges-Köhler-Allee 201, 79110 Freiburg, Lab room

Experiment 4: Schänzlestrasse 1, Di Ventura Lab, room 04.023 (4thfloor), teaching room 045 (ground floor)

Time: SS
Participants: max. 9
Group size: max. 3 students per setup

Description

Aim of this practical course is to provide students with an overview and introduction to standard optogenetic approaches. These include four experiments: 1) Histological analysis of cryosections with a focus on cortical areas 2) Two-Photon Ca2+ Imaging and Optogenetics in Organotypical Slice Cultures 3) Extracellular electrophysiology combined with optogenetics 4) Gibson assembly-based cloning of a reporter system regulated by a light-inducible synthetic transcription factor (TF) in mammalian cells.

Impressions from SS 2022

Contact person (implementation)

Prof. Dr.

Ilka Diester

Head

+49 (0)761 203-8440
ilka.diester@biologie.uni-freiburg.de

Contact person (implementation)

Priv.-Doz. Dr.

Philippe Coulon

Senior Research Scientist

+49 (0)761 203-98637
philippe.coulon@biologie.uni-freiburg.de
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